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DOTY
4 mm H/X/Y MAS probe used for
separated local-field experiments on
membrane systems at natural isotopic abundance.
Solid state separated local-field DROSS experiments
show differences in 13C chemical shifts and 13C-1H residual dipolar
couplings, used
to determine segmental order parameters. For POPC/EYSM/Chol (1:1:1)
membranes, these structural results provide valuable insight into
determinants of raft-like phase behavior and membrane lipid structure.
Figure 1. (a) 2D DROSS spectra; (b) Solid-state 13C chemical shifts
and residual dipolar coupling lineshapes from the DROSS experiment.
This ternary membrane system was at natural isotopic abundance,
liquid crystalline phase, and a sample temperature of 48oC. Data
was collected with a Doty 4 mm H/X/Y XC4 MAS probe used in a 500
MHz narrow bore NMR magnet.
Data courtesy of Avigdor Leftin, Klaus Beyer, and Michael F. Brown;
Univ. of Arizona.
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